论文部分内容阅读
目的 探讨高敏感性Kras基因点突变检测方法在肺癌临床的应用价值。方法 应用二步(巢式)聚合酶链反应结合限制性片断多态性分析方法(PCRRFLP)检测临床肺癌标本Kras基因第12密码子点突变。结果 二步PCRRFLP法检测Kras点突变的敏感性较一步法提高约64倍。55例肺癌石蜡包埋标本中,Kras点突变检出率为47.3%;对另67例纤支镜标本进行基因检测,以Kras突变进行基因诊断的敏感性为70.9%,特异性为91.7%。结论 二步PCRRFLP法对临床肺癌标本Kras点突变检出率较高,对肺癌有一定的辅助诊断价值
Objective To explore the clinical application value of high sensitive Kras gene point mutation detection method. Methods Two-step (nested) polymerase chain reaction combined with restriction fragment polymorphism (PCRRFLP) was used to detect point mutations in codon 12 of Kras gene in clinical lung cancer specimens. Results The sensitivity of the two-step PCRRFLP method for detecting the point mutation of Kras was about 64-fold higher than that of the one-step method. In 55 paraffin-embedded specimens of lung cancer, the detection rate of Kras point mutation was 47.3%. Another 67 specimens of bronchoscopic specimens were genetically tested. The sensitivity of the Kras mutation to gene diagnosis was 70.9% and the specificity was 91.7%. Conclusion Two-step PCRRFLP method has higher detection rate of Kras point mutation in clinical lung cancer specimens, and has certain auxiliary diagnostic value for lung cancer.