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目的探讨北方地区汉族人多发性骨髓瘤(MM)与人类主要组织相容性复合体MHC-DRB1*基因多态性的关系。方法应用顺序特异性引物和聚合酶链反应(PCR-SSP)技术,对21例多发性骨髓瘤患者及32例无血缘关系的健康人的MHC-DRB1*各等位基因及亚基因进行了检测分析,并将该方法与其它检测MHC等位基因的方法进行对比。结果结果表明,MHC-DRB1*11(RR=2.36,P<0.05;MHC-DRB1*11基因与MM呈正相关,显著高于对照组,两组之间比较差异有显著性意义,而其它MHC-DRB1*各等位基因未见异常,均无统计学差异。结论本项研究结果提示,MHC-DRB1*11基因可能是我国北方汉族人MM致病的易感基因,为揭示MM的发病机制中免疫遗传学作用提供了重要信息和依据。
Objective To investigate the association between multiple myeloma (MM) in Han population and MHC-DRB1 * polymorphism in human major histocompatibility complex. Methods Sequence-specific primers and polymerase chain reaction (PCR-SSP) were used to detect the alleles and subgenotypes of MHC-DRB1 * in 21 patients with multiple myeloma and 32 healthy unrelated individuals The method was compared with other methods to detect MHC alleles. The results showed that MHC-DRB1 * 11 (RR = 2.36, P <0.05); MHC-DRB1 * 11 gene was positively correlated with MM and significantly higher than the control group, the difference between the two groups was significant, DRB1 * alleles showed no abnormality.Conclusion The results of this study suggest that MHC-DRB1 * 11 may be a susceptibility gene for MM in northern Han Chinese. To reveal the pathogenesis of MM The role of immune genetics provides important information and basis.