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目的:探讨太空环境对甘草酸相关基因的诱变作用分析。方法:利用返回式卫星搭载甘草,18 d后返回地球,搭载种子返回地面后与对照组同时种植于实验田中,采集三年生甘草叶子,对甘草酸形成相关基因ITS序列,β-香树酯醇合成酶基因的多态性以及基因表达差异进行了研究。结果:甘草经过卫星搭载后,甘草酸相关基因ITS序列没有发生变化,而甘草酸合成关键基因β-香树酯醇合成酶基因某些位点呈现了差异性,并且太空环境对甘草酸合成关键基因β-香树酯醇合成酶基因表达有一定影响。结论:太空环境对甘草酸相关形成和表达产生一定影响,这些变化对于揭示太空环境对甘草酸形成的基因机制具有重要意义,并且对于后期选育甘草优良种质奠定基础。
Objective: To investigate the mutagenic effects of space environment on glycyrrhizin-related genes. Methods: The return satellites were loaded with Glycyrrhiza uralensis and returned to earth after 18 days. The seeds were returned to the ground and planted in the experimental field at the same time with the control group. The three-year-old licorice leaves were collected to analyze the ITS sequence of glycyrrhizin- Polymorphisms of synthetase genes and their differences in gene expression were studied. Results: The ITS sequence of glycyrrhizin-related genes did not change after the satellite was loaded with Glycyrrhiza uralensis, and some sites of the β-misutyl alcohol synthetase gene of glycyrrhizin synthesis were different, and space environment had the key to glycyrrhizin synthesis Gene β-misgregate alcohol synthase gene expression has a certain impact. CONCLUSIONS: The space environment has certain influence on the formation and expression of glycyrrhizin. These changes are of great significance for revealing the genetic mechanism of glycyrrhizin formation in space environment, and lay a foundation for the breeding of excellent germplasm of Glycyrrhiza uralensis later.