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目的:探讨药理筛选在完善以有效成分的正交实验结果为依据所确立的蒿鳖养阴软坚方制剂工艺中的作用。方法:以复方中辅药的水溶性(丹酚酸B、梓醇)和脂溶性(大黄素)有效成分为评价指标,按HPLC测定其含量,对溶剂倍量、提取次数、提取时间、乙醇浓度等因素各3个水平进行正交实验,据以制定提取工艺共10种方案。0.5mlCCl4/100gwt皮下注射16次(后15次注射用CCl4:橄榄油=4:6,V/V)辅以10%乙醇为饮料引起大鼠肝纤维化,成模后按体重随机区组法分组,分别以蒿鳖养阴软坚方10种提取方案各2个剂量(24.48g/kg,8.16g/kg)治疗用药,每日1次、每周6天灌胃共2个月,模型对照组和正常对照组则灌胃蒸馏水。以肝胶原蛋白含量、肝组织形态及血清中肝纤维化标志物评价疗效。结果:蒿鳖养阴软坚方按方案1-1(水提低含量和醇提低含量)提取,24.48g/kg能明显降低肝胶原蛋白及血清透明质酸和IV型胶原水平(P<0.05),按方案3-2(水提低含量和醇提高含量)提取,8.16g/kg能降低肝胶原蛋白及明显降低血清透明质酸和IV型胶原水平(P<0.05)。结论:对有效成分含量的正交实验基础上以疗效指标进行药理筛选应作为确立复方提取工艺方案的最终依据。
OBJECTIVE: To explore the role of pharmacological screening in perfecting the technology of Artemisia annua soft nourishing and softening herbs established on the basis of orthogonal experimental results of active ingredients. Methods: The water-soluble (salvianolic acid B, catalpol) and fat-soluble (emodin) active ingredients of the compound auxiliary drugs were used as the evaluation indexes. The content of the compound was determined by HPLC. The effects of solvent doubling times, extraction times, Concentration and other factors in each of the three levels of orthogonal experiments, extraction process to develop a total of 10 programs. 0.5mlCCl4 / 100gwt subcutaneous injection of 16 times (15 times after injection of CCl4: olive oil = 4: 6, V / V) supplemented with 10% ethanol as the beverage induced liver fibrosis, The rats were divided into two groups (control group, n = 10) and control group (n = 10). The treatment groups were given 2 doses of 24.48g / kg and 8.16g / Group and normal control group were fed distilled water. The effect of liver collagen content, liver morphology and serum markers of liver fibrosis were evaluated. Results: Artemisia turtle Yangyinjianjian extract according to the program 1-1 (water content and alcohol content) extract, 24.48g / kg can significantly reduce the level of hepatic collagen and serum hyaluronic acid and collagen IV (P <0.05 ), According to the program 3-2 (water content and alcohol content increased), 8.16g / kg can reduce hepatic collagen and significantly lower serum hyaluronic acid and type IV collagen (P <0.05). Conclusion: Based on the orthogonal experiment of active ingredients, pharmacological screening based on efficacy index should be used as the final basis for establishing compound extraction process.