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[Objective] To determine the content of quercetin in Fufang Luobuma granule. [Method] RP-HPLC was adopted to detect the content. Chromatographic column was Zorbax-C18(250 mm × 4.6 mm,5 μm); mobile phase was anhydrous methanol-0.5% phosphoric acid (V/V, 50∶ 50); flow rate was 1.0 ml/min; detection wavelength was 370 nm; column temperature was 30 ℃; and injection volume was 10 μl. [Result] The average recovery rate of quercetin was 100.2% and the RSD was 1.94%(n=5). The quercetin content in Fufang Luobuma granule (sugar) was 0.729 5 mg/g, and that in Fufang Luobuma granule (sugar free) was 0.955 mg/g. [Conclusion] This method was simple, accurate, and repeatable, and could be used for the content determination of quercetin in Fufang Luobuma granule.
[Objective] To determine the content of quercetin in Fufang Luobuma granule. [Method] RP-HPLC was detected to detect the content. Chromatographic column was Zorbax- C18 (250 mm × 4.6 mm, 5 μm); mobile phase was anhydrous methanol- 0.5% phosphoric acid (V / V, 50:50); flow rate was 1.0 ml / min; detection wavelength was 370 nm; column temperature was 30 ° C .; and injection volume was 10 μl. [Result] The average recovery rate of quercetin was 100.2% and the RSD was 1.94% (n = 5). The quercetin content in Fufang Luobuma granule (sugar) was 0.729 5 mg / g, and that in Fufang Luobuma granule (sugar free) was 0.955 mg / g. ] This method was simple, accurate, and repeatable, and could be used for the content determination of quercetin in Fufang Luobuma granule.