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目的研究大明胶囊(Daming capsule,DM)对链脲佐菌素(streptozocin,STZ)诱导的1型糖尿病大鼠的血糖影响。方法将65只成年雄性Sprague-Dawley(SD)大鼠随机分为5组:DM低(50 mg·kg-1·d-1)、中(100 mg·kg-1·d-1)、高(200 mg·kg-1·d-1)剂量组、空白组及模型组。灌胃给予大鼠不同浓度的DM,空白组和模型组给予同等体积的生理盐水,每天两次。两周后,腹腔注射STZ 65 mg·kg-1建立1型糖尿病模型。STZ注射后3天、7天检测大鼠空腹血糖,1个月后检测糖耐量。苏木素-伊红(HE)染色和末端脱氧核糖核苷酸转移酶介导的缺口末端标记法(TUNEL)观察各组胰岛形态学和凋亡。结果 DM显著降低STZ诱导糖尿病大鼠的空腹血糖值并改善糖尿病大鼠的糖耐量;在STZ大鼠,HE染色显示DM升高STZ大鼠胰岛的数量;TUNEL染色结果显示DM组的胰岛β细胞凋亡较STZ模型组显著减少。结论预防性给予DM能降低STZ诱导的1型糖尿病大鼠空腹血糖并明显改善糖耐量,减少胰岛β细胞凋亡。
Objective To investigate the effect of Daming capsule (DM) on the blood glucose of type 1 diabetic rats induced by streptozocin (STZ). Methods Sixty-five adult male Sprague-Dawley rats were randomly divided into 5 groups: DM (50 mg · kg -1 · d -1), medium (100 mg · kg -1 · d -1), high (200 mg · kg-1 · d-1) dose group, blank group and model group. Gavage given different concentrations of DM rats, blank group and model group given the same volume of saline twice a day. Two weeks later, type 1 diabetic model was established by intraperitoneal injection of STZ 65 mg · kg-1. Fasting blood glucose was measured at 3 days and 7 days after STZ injection, and glucose tolerance was measured after 1 month. Hematoxylin and eosin (HE) staining and terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL) were used to observe islet morphology and apoptosis in each group. Results DM significantly decreased fasting blood glucose (FBG) and glucose tolerance in STZ-induced diabetic rats. In STZ rats, HE staining showed that DM increased the number of islets in STZ rats. TUNEL staining showed that pancreatic β-cells Apoptosis than STZ model group significantly reduced. Conclusion Preventive administration of DM can reduce fasting blood glucose (FBG) and improve glucose tolerance (IGT) in STZ-induced type 1 diabetic rats and decrease pancreatic β-cell apoptosis.