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As a kind of mutagen,ion beam irradiation can create abundant biological mutations.A population of about 2000 lines was generated by irradiating dry wheat seeds of XiaoYan 81with low-energy nitrogen ion beams.The traits of the plant,such as height,spike type,fertility,stem color and awn length,were investigated.The mutation rate in terms of the plant height inM_2 was 2.9%.Eighteen deletion mutants of TaGA2ox1 were obtained.Associate analysis showedthat TaGA2ox1 was closely related to the plant height.Most of the TaGA2ox1-deleted mutantswere higher than the control,suggesting that the biological function of TaGA2ox1 is similar toits homologues in other plants.These results demonstrate that ion beam irradiation is an efficienttool in the construction of a mutant library for wheat.
As a kind of mutagen, ion beam irradiation can create abundant biological mutations. A population of about 2000 lines was generated by irradiating dry wheat seeds of Xiao Yan 81 with low-energy nitrogen ion beams.The traits of the plant, such as height, spike type , fertility, stem color and awn length, were investigated. The mutation rate in terms of the plant height inM_2 was 2.9% .Eighteen deletion mutants of TaGA2ox1 were obtained. Associated analysis showed that TaGA2ox1 was closely related to the plant height. -deleted mutantswere higher than the control, suggesting that the biological function of TaGA2ox1 is similar toits homologues in other plants. These results demonstrate that ion beam irradiation is an efficienttool in the construction of a mutant library for wheat.