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为选育优质花椰菜新品种,指导种质资源引进和利用,本研究采用简单重复序(simple sequence repeat,SSR)标记和相关序列扩增多态性(sequence-related amplified polymorphism,SRAP)标记对38份花椰菜自交系进行了遗传多样性分析,分别从48对SSR引物、48对SRAP引物中各筛选出4对有效引物。4对SSR引物扩增的总条带数为47个,多态性条带为39个,平均多态性比率达83.0%;4对SRAP引物扩增的总条带数为86个,多态性条带为51个,平均多态性比率为59.3%,该结果显示花椰菜自交系间具有较丰富的遗传多样性。UPGMA聚类分析揭示了花椰菜自交系的熟期与其遗传差异相关。
In order to breed new varieties of high quality cauliflower and guide the introduction and utilization of germplasm resources, we used simple sequence repeat (SSR) markers and sequence-related amplified polymorphism (SRAP) The genetic diversity of cauliflower inbred lines was analyzed. Four effective primers were screened out from 48 pairs of SSR primers and 48 pairs of SRAP primers respectively. Four pairs of SSR primers amplified 47 total bands and 39 polymorphic bands with an average of 83.0%. The total number of bands amplified by 4 pairs of SRAP primers was 86, There were 51 sexual bands with the average polymorphism rate of 59.3%. The results showed that cauliflower had a rich genetic diversity among inbred lines. UPGMA cluster analysis revealed that the maturity of cauliflower inbred lines correlated with their genetic differences.