目的分析淡色库蚊抗性品系、敏感品系、微山湖现场品系蚊体内酚氧化酶基因表达量。方法根据酚氧化酶基因序列设计引物扩增基因片段,荧光定量PCR检测不同品系淡色库蚊体内的酚氧化酶基因表达水平差异。结果酚氧化酶在昆虫的正常发育过程中具有重要的生理作用,酚氧化酶基因在抗性品系蚊体内的表达量是敏感品系的6.74倍,微山湖现场品系是敏感品系的4.19倍。结论淡色库蚊酚氧化酶基因可做为新的抗性检测及治理基因靶标。 Objective To analyze the expression of phenoloxidase gene in Culex pipiens pallens resistance strain, susceptible strain and Weishanhu site. Methods According to the sequence of phenoloxidase gene, primers were designed to amplify the gene fragments. Fluorescent quantitative PCR was used to detect the difference of phenoloxidase gene expression among different strains of Culex. Results Phenoloxidase had an important physiological role in the normal development of insects. The expression level of phenoloxidase gene in mosquitoes was 6.74 times of that of susceptible strains and 4.19 times of that of sensitive strains in Weishan Lake. Conclusion The results showed that the phenoloxidase gene from Culex pipiens pallens could be used as a new gene target for resistance testing and management.