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目的检测结肠黏液腺癌和正常结肠黏膜组织中CD44v6和Survivin的表达,分析二者的表达差别及相关性。方法 43例结肠黏液腺癌术后留取的新鲜组织为观察组,非肿瘤性结肠黏膜新鲜组织23例为对照组,均制备单细胞悬液后应用流式细胞术检测两组CD44v6和Survivin表达。结果两组CD44v6和Survivin表达量差异有统计学意义(P<0.05),观察组CD44v6和Survivin表达量与肿瘤最大径、淋巴结转移和增殖指数均密切相关,CD44v6的表达与肿瘤是否浸出肌层相关。线性相关分析显示观察组中CD44v6和Survivin呈正相关。结论结肠黏液腺癌中CD44v6和Survivin的表达异常,不仅可以促进病变形成,还对病变进展有重要作用。二者可能具有正向协同作用。
Objective To detect the expression of CD44v6 and Survivin in colonic mucinous adenocarcinoma and normal colonic mucosa, and to analyze the difference and correlation between them. Methods The fresh tissue collected from 43 cases of colonic mucinous adenocarcinoma after operation was observed, and 23 cases of non-neoplastic colonic mucosa fresh tissue were taken as control group. After preparing single cell suspension, flow cytometry was used to detect the expression of CD44v6 and Survivin . Results The expressions of CD44v6 and Survivin in the two groups were significantly different (P <0.05). The expressions of CD44v6 and Survivin in the observation group were closely related to the maximum diameter of tumor, lymph node metastasis and proliferation index, and the expression of CD44v6 correlated with the leaching of myometrium . Linear correlation analysis showed that there was a positive correlation between CD44v6 and Survivin in observation group. Conclusion The abnormal expression of CD44v6 and Survivin in colonic mucinous adenocarcinoma can not only promote the formation of the lesion, but also play an important role in the progression of the lesion. Both may have positive synergy.