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目的:测定国内不同产地蜂胶中白杨素的含量。方法:采用HPLC法,Diamonsil(钻石)C18(46mm×250mm,5μm)色谱柱,甲醇-0.4%的磷酸水(65∶35)为流动相,流速为1.0mL/min,检测波长为256nm。结果:白杨素在0.00592~0.03552mg/mL范围内呈良好的线性关系(R2=0.9999);白杨素的含量,山东最高,为23.054mg.g-1,其次是内蒙和河南,分别为14.170mg.g-1,13.656mg.g-1,甘肃最低为6.697mg.g-1。结论:不同产地蜂胶中白杨素的含量有一定差异。
Objective: To determine the content of chrysin in propolis from different regions in China. METHODS: The HPLC method was used on a Diamonsil C18 (46mm×250mm, 5μm) column with methanol-0.4% phosphoric acid (65:35) as the mobile phase. The flow rate was 1.0 mL/min and the detection wavelength was 256 nm. Results: The chrysin had a good linear relationship in the range of 0.00592-0.03552 mg/mL (R2=0.9999). The content of chrysin was the highest in Shandong, which was 23.054 mg.g-1, followed by Inner Mongolia and Henan, which were 14.170 mg respectively. The lowest level of .g-1, 13.656mg.g-1 in Gansu was 6.697mg.g-1. Conclusion: The content of chrysin in propolis from different areas has some differences.