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【目的】研究缺氧缺血(HI)对新生大鼠脑白质细胞血管内皮生长因子(VEGF)及诱导型一氧化氮合酶(iNOS)表达的影响,探讨VEGF及iNOS在新生大鼠脑白质损伤(WMD)发病机制中的作用。【方法】将3日龄大鼠随机分为实验组及假手术组,建立新生大鼠WMD模型,分别予HI后12、24、48、72h及7d处死,对其脑组织取材后行HE染色观察其病理改变,并应用免疫组织化学法检测其VEGF及iNOS的表达水平。【结果】在新生大鼠脑白质中,1)VEGF表达水平予HI后12h开始明显上升,24h后达高峰,至7d恢复正常,与对照组比较,实验组脑室周围白质VEGF的表达在12、24、48、72h有统计学意义。2)实验组iNOS于HI后12h表达开始增加,72h达高峰,7d仍有表达,与对照组比较,差异有统计学意义。【结论】HI可导致新生大鼠脑白质VEGF及iNOS的表达水平显著增高,参与新生大鼠WMD的病理生理过程。
【Objective】 To investigate the effect of hypoxia-ischemia (HI) on the expression of vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) in the neutrophilic white matter of neonatal rats and to explore the role of VEGF and iNOS in the brain white matter The role of injury (WMD) in the pathogenesis. 【Methods】 The 3-day-old rats were randomly divided into experimental group and sham-operated group, and the WMD model of neonatal rats was established. The rats were sacrificed at 12, 24, 48, 72 and 7 days after HI, The pathological changes were observed, and the expression of VEGF and iNOS were detected by immunohistochemistry. 【Results】 The expression of VEGF in brain tissue of neonatal rats increased significantly at 12h after HI and peaked at 24h and returned to normal after 7d. Compared with the control group, the expression of VEGF in periventricular white matter in experimental group was 12, 24,48,72 h was statistically significant. 2) The expression of iNOS in experimental group began to increase 12h after HI, peaked at 72h, and still remained at 7d. The difference was statistically significant compared with the control group. 【Conclusion】 HI can cause the expression of VEGF and iNOS in the white matter of neonatal rats to be significantly increased, which is involved in the pathophysiological process of WMD in neonatal rats.