论文部分内容阅读
研究了茎尖大小、激素浓度、培养基成分对药用植物掌叶半夏脱毒效果的影响以及影响无病毒苗快速繁殖的因素等,结果表明:茎尖大小是影响茎尖成活和脱毒效果的主要因素,0.2~0.5mm的茎尖培养在MS+6-BA 1.0mg/L+NAA 0.1mg/L+蔗糖30g/L+琼脂7g/L培养基上,成活率为53.3%,病毒脱除率为76.7%,培养基中附加247mg/L NH_4~+可有效提高茎尖成活率至86.7%,同种培养基上继续培养,可一次性成功诱导形成试管苗,移栽成活率高达100%。MS+6-BA 2.0mg/L+NAA 0.5mg/L+GA30.5mg/L组合最有利于试管茎的形成和发育,从而建立了以掌叶半夏微茎尖为外植体的脱毒和快速繁殖体系。
The effects of stem tip size, hormone concentration and medium composition on the detoxification effect of medicinal plant Paeonia lactiflora and the factors influencing the rapid reproduction of the virus-free seedling were studied. The results showed that the size of the shoot tip was the effect of survival and detoxification The main factor was 0.2-0.5 mm shoot tip cultured in MS + 6-BA 1.0mg / L + NAA 0.1mg / L + sucrose 30g / L + agar 7g / L medium, the survival rate was 53.3%, the virus removal rate 76.7%. Adding 247mg / L NH_4 ~ + to the culture medium can effectively improve the survival rate of shoot tip to 86.7%, and continue to cultivate on the same medium. Successfully induce the formation of in vitro plantlets with 100% survival rate. MS + 6-BA 2.0mg / L + NAA 0.5mg / L + GA30.5mg / L combination is most conducive to the formation and development of test tube stem, thus establishing a drug-sensitive and rapid Reproduction system.