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目的探讨人转化生长因子β１（ＴＧＦβ１）正、反义基因对人肝癌细胞 ＳＭＭＣ－７７２１增殖的影响。方法将ＴＧＦβ１正、反义基因分别导入ＳＭＭＣ－７７２１细胞，观察各转染细胞生长情况、凋亡特征及Ｆａｓ、ｐ５３的表达。结果与空质粒转染细胞相比，正义ＴＧＦβ１基因转染细胞生长速度减慢，出现明显的细胞凋亡形态学改变，３－羟基末端标记法（ＴＵＮＥＬ）为阳性，而反义基因转染细胞未发生上述改变，两者都可检测到ｐ５３信号，但未检测到Ｆａｓ表达。结论ＴＧＦβ１可通过诱导ＳＭＭＣ－７７２１凋亡而对其生长起负调控作用，其机制可能与Ｆａｓ／ＦａｓＬ、ｐ５３无关。
Objective To investigate the effect of human transforming growth factor β1 (TGFβ1) positive and antisense genes on the proliferation of human hepatocellular carcinoma cell line SMMC-7721. Methods The positive and antisense TGFβ1 genes were respectively introduced into SMMC-7721 cells, and the growth, apoptosis and expression of Fas and p53 in the transfected cells were observed. Results Compared with the empty plasmid transfected cells, the sense TGFβ1 gene transfected cells had a slower growth rate and obvious morphological changes in apoptosis. The TUNEL was positive and the antisense gene transfected cells. No such change occurred, both detected p53 signal, but no Fas expression was detected. Conclusion TGFβ1 can negatively regulate the growth of SMMC-7721 cells by inducing apoptosis, and the mechanism may be independent of Fas/FasL and p53.