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首次提出了用猪小腿皮中提取的胶原纤维作载体，采用电解包埋并辅以戊二醛交联的酶固定化方法制备胆固醇氧化酶膜，并与氧电极配合制备了游离胆固醇单酶传感器．在所选择的最佳试验条件下用于血样分析，对游离胆固醇的测定结果，与酶法测定总胆固醇值范围相符
It is the first time to use the collagen fibers extracted from the calf skin of pigs as the carrier. The cholesterol oxidase membrane was prepared by the enzymatic immobilization method with electrolytic embedding and glutaraldehyde cross-linking. The free cholesterol mono-enzyme sensor . In the best choice of the experimental conditions for blood samples for the determination of free cholesterol, and enzymatic determination of total cholesterol value range