论文部分内容阅读
目的 探讨保定地区 HFRS流行高峰的原因。方法 对 HFRS病人急性期血清用 RT- PCR方法进行病毒基因分型 ,从 1例家鼠型 (SEO型 )汉坦病毒感染的病人血清中分离病毒 ,并进行核苷酸序列测定。结果 31例病人中 ,2例为姬鼠型(HTN型 ) ,2 4例为家鼠型 (SEO型 ) ,1例 SEO型病毒经分离鉴定测序后与 SEOU L、R2 2 株进行比较 ,M基因核苷酸同源性分别为 :95 .15 %、93.94% ;氨基酸同源性均为 97.2 7%。结论 所分离 SEO型汉坦病毒为 1996年流行高峰年的主要流行株 ,未发现病毒基因片段的重排 ,流行不是由病毒基因重排引起。不同地区 SEO型病毒分子结构差异较小 ,较为稳定
Objective To explore the causes of HFRS epidemic peak in Baoding. Methods The virus was genotyped by RT-PCR in the serum of patients with HFRS in the acute phase. The virus was isolated from the serum of one Hantaan-infected patient (SEO type) and the nucleotide sequence was determined. RESULTS: Of the 31 patients, 2 were of the Apodemus type (HTN) and 24 were of the Domestic type (SEO type). One SEO virus was isolated and identified with SEOU L and R2 2 strains. M The nucleotide homology of the gene was 95.15% and 93.94%, respectively. The amino acid homology was 97.27%. Conclusion The SEO-type Hantavirus isolated was the main epidemic peak in the peak of 1996 epidemic. No rearrangement of the viral gene was found, and the epidemic was not caused by viral gene rearrangement. Different regions of the SEO virus molecular structure of the smaller, more stable