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目的:观察人参皂苷Rg1、Rb1及其联合使用对树突状细胞系DC2.4吞噬抗原和表面分子表达的影响。方法:体外培养DC2.4细胞,分别加入不同剂量人参皂苷Rg1、Rb1及Rg1+Rb1孵育24h后,分别加入异硫氰酸荧光素标记的卵白蛋白(FITC-OVA),流式细胞仪检测DC2.4细胞吞噬卵白蛋白抗原的情况;体外培养DC2.4细胞,分别加入LPS和不同剂量人参皂苷孵育24h,流式细胞仪检测细胞表面分子CD40的表达。结果:人参皂苷Rg1、Rb1、Rg1+Rb1在一定浓度可促进DC2.4细胞抗原吞噬的能力;三者均抑制CD40的表达。结论:人参皂苷Rg1、Rb1可通过影响DCs的抗原吞噬功能来发挥免疫调节作用;Rg1、Rb1联合使用与单独应用比较无明显差异。
Objective: To observe the effects of ginsenoside Rg1, Rb1 and their combination on phagocytic antigen and surface molecule expression of dendritic cell line DC2.4. Methods: DC2.4 cells were cultured in vitro. After incubated with different doses of ginsenoside Rg1, Rb1 and Rg1 + Rb1 for 24 h respectively, fluorescein isothiocyanate labeled ovalbumin (FITC-OVA) was added and flow cytometry was used to detect DC2 .4 cells phagocytosed ovalbumin antigen; cultured in vitro DC2.4 cells were added LPS and different doses of ginsenoside incubated 24h, flow cytometry to detect the expression of cell surface molecules CD40. Results: Ginsenosides Rg1, Rb1 and Rg1 + Rb1 could promote the phagocytosis of DC2.4 cells at a certain concentration. All three inhibited the expression of CD40. Conclusion: Ginsenosides Rg1 and Rb1 can play an immunomodulatory role through affecting the antigen phagocytosis of DCs. The combination of Rg1 and Rb1 has no significant difference compared with the single application.