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目的分析输入性麻疹野病毒对云南省边境地区麻疹分子流行病学的影响。方法用淋巴信号激活因子转染的非洲绿猴肾细胞(Vero/SLAM细胞),从麻疹患者的标本中分离麻疹病毒,用逆转录-聚合酶链反应(RT-PCR)扩增核蛋白(Nucleoprotein,N)基因羧基末端的676个核苷酸片段,对扩增产物进行核苷酸序列测定。并以N基因羧基末端450个核苷酸序列构建基因亲缘性关系树,进行核苷酸变异分析。结果云南省于2009年监测到麻疹病毒株MVi/Menglian.Yunnan.CHN/47.09/1,经过世界卫生组织(WHO)认定为D11基因型,为全球首次发现,并将此毒株定为D11基因型的标准株;云南省于2012年监测到麻疹病毒株MVi/Yunnan.CHN/02.12/01,在亲缘性关系树上与D9基因型参考株同在一个大的分支,同源性为96.3%,为D9基因型麻疹病毒。两起麻疹疫情均为输入性麻疹野病毒。结论两次输入性麻疹病毒均造成云南省边境地区的流行,云南省在含麻疹成分疫苗的常规免疫基础上适时进行强化免疫,有效阻断了输入麻疹病毒的传播。
Objective To analyze the influence of measles wild measles virus on the molecular epidemiology of measles in the border area of Yunnan Province. Methods Vero / SLAM cells transfected with lymphotoxin-activated factor were used to isolate measles virus from measles patients. Nucleoprotein was amplified by reverse transcription-polymerase chain reaction (RT-PCR) , N) gene carboxy terminal 676 nucleotide fragments of the amplification product nucleotide sequence determination. Nucleotide sequence analysis of 450 nucleotide sequences of the N gene was used to construct the phylogenetic tree. Results The measles virus strain MVi / Menglian.Yunnan.CHN / 47.09 / 1 was detected in Yunnan Province in 2009 and was identified as the D11 genotype by the World Health Organization (WHO). It was the first discovery in the world and the D11 gene . The measles virus strain MVi / Yunnan.CHN / 02.12 / 01 was detected in Yunnan Province in 2012. The homology was 96.3% with the D9 genotype reference strain in the phylogenetic tree, , For the D9 genotype measles virus. The two outbreaks of measles were imported wild measles virus. Conclusions Both imported measles virus caused the epidemic in border areas of Yunnan Province. Yunnan Province, on the basis of routine immunization with measles vaccine, timely and intensively immunized, effectively blocking the transmission of imported measles virus.