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采用单细胞凝胶电泳技术研究了富硒金针菇中提取的硒多糖对铬致小鼠骨髓细胞DNA损伤的保护作用,进而通过考察小鼠内源性脂质过氧化的抑制作用进一步探究硒多糖的生物活性.结果表明:硒多糖在硒含量为0.0244～0.0650μg.mL-1范围内,随浓度和在小鼠体内作用时间的增加,骨髓细胞尾长和尾矩与对照组相比都有显著下降;对小鼠内源性脂质过氧化的抑制作用随浓度的升高而增强,与对照组相比有显著差异.硒多糖作为有机硒对铬致细胞DNA损伤和脂质过氧化均有保护作用,可为新型有机硒补剂的开发和有机硒在食品、药品以及预防科学领域的应用提供科学的理论依据.
Single cell gel electrophoresis was used to study the protective effect of selenium polysaccharide extracted from selenium-enriched Flammulina velutipes against DNA damage of bone marrow cells induced by chrome in mice and to further explore the effect of endogenous lipid peroxidation in mouse on selenium polysaccharide Biological activity.The results showed that the tail length and tail moment of selenium polysaccharide in the range of 0.0244 ~ 0.0650μg.mL-1 with concentration and time in mice increased significantly compared with the control group Decreased.The inhibitory effect of endogenous lipid peroxidation in mice increased with the increase of concentration, which was significantly different from the control group.Selenium polysaccharides as organic selenium had a significant effect on DNA damage and lipid peroxidation It can provide a scientific theoretical basis for the development of new organic selenium supplements and the application of organic selenium in food, medicine and prevention science.