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目的:通过研究芦丁及芹菜中的总黄酮对B-Z化学振荡体系的扰动行为,为芦丁及总黄酮含量的测定提供一种简便快速的新方法。方法:以芦丁纯品和芹菜中的总黄酮提取液为振荡底物和扰动成分,在振荡反应的不同时期分别加入一定浓度的芦丁水溶液,考察其对振荡谱图及振荡参数的扰动规律,同时采用B-Z振荡实验装置记录化学振荡体系中电位E随时间t的变化值。结果:在最优测试条件下(搅速500 r·min~(-1),反应温度37℃),在波峰位置加入芦丁,根据不同浓度芦丁对振荡参数的扰动规律,发现在8.0×10~(-3)~2.6×10~(-2)g·L~(-1),芦丁质量浓度与振荡周期的改变量(ΔT)存在良好的线性关系,其线性方程为ΔT(s)=699.4×[芦丁质量浓度]+4.5,相关系数为0.996 8,检测限为1.0×10~(-7)g·L~(-1)(S/N=3)。基于芦丁建立的标准曲线,利用B-Z振荡体系对芹菜提取液中的总黄酮含量进行了测定,其结果与紫外分光光度法基本一致。结论:该研究建立了一种测定芦丁及芹菜中总黄酮含量的化学振荡新方法,该方法具有耗时短、操作简单、仪器成本低的优点。
OBJECTIVE: To study the perturbation behavior of total flavonoids in rutin and celery on the chemical oscillation system of B-Z, and to provide a simple and rapid new method for the determination of rutin and total flavonoids. Methods: The total flavonoids extract of Rutin puree and celery was used as oscillating substrate and perturbation component. A certain concentration of rutin aqueous solution was added at different periods of shaking reaction to investigate the perturbation law of oscillation spectrum and oscillation parameters. Meanwhile, BZ oscillation experimental device is used to record the change of potential E with time t in chemical oscillation system. Results: Rutin was added at the peak position under the optimum conditions (stirring speed 500 r · min -1, reaction temperature 37 ℃). According to the perturbation law of the oscillation parameters of rutin with different concentrations, There was a good linear relationship between the mass concentration of rutin and the change of the oscillation period (ΔT) in the range of 10 -3 to 2.6 × 10 -2 g · L -1. The linear equation was ΔT (s ) = 699.4 × [concentration of rutin] +4.5, the correlation coefficient was 0.996 8, the detection limit was 1.0 × 10 -7 g · L -1 (S / N = 3). Based on the standard curve of rutin, the content of total flavonoids in celery extract was determined by B-Z oscillation system. The results were in good agreement with those of UV spectrophotometry. Conclusion: This study established a new method for chemical oscillation determination of total flavonoids in rutin and celery. The method has the advantages of short time-consuming, simple operation and low instrument cost.