论文部分内容阅读
Objective:To investigate the effect of modified Xiaochaihu Decoction(小柴胡汤,MXD)on transforming growth factor-β1/Sma-and Mad-related proteins(TGF-βl/Smads)signaling pathway in rats with chronic pancreatitis(CP)induced by dibutyltin dichloride.Methods:Thirty healthy male Wistar rats were randomly divided into the normal control group,CP group and CP+MXD-treated group.CP was induced by injection of dibutyitin dichloride(DBTC,7 mg/kg of body weight)into the right caudal vein,and the control rats were treated with vehicle.MXD was given daily by gavage at a dose of 10 g/kg of body weight,starting from the day after CP induction.After 28-day treatment,the n-benzoyl-tyrosyl para-aminobenzoic acid(NBT-PABA)test was carried out to evaluate exocrine pancreatic function.Then,rats were sacrificed,and pancreatic tissues were harvested for histological evaluation.In addition,the mRNA expression of TGF-13 1,TGF-β1 typeⅡreceptor(TGFβRⅡ),Smad3 and Smad7 was determined in pancreatic tissues by using real-time polymerase chain reaction.Results:Treatment of CP with MXD improved the PABA recovery,decreased the histological lesion,and reduced the mRNA expression of TGF-β1,TGFβRⅡand Smad3(P<0.05).However,MXD had no effect on Smad7 mRNA level.Conclusions:MXD could protect the pancreas against chronic injury and improve pancreatic exocrine function in DBTC induced rat CP model.Its mechanism may involve inhibition of the TGF-β1/Smads signaling pathway.
Objective: To investigate the effect of modified Xiaochaihu Decoction on transforming growth factor-β1 / Sma-and Mad-related proteins (TGF-β1 / Smads) signaling pathway in rats with chronic pancreatitis (CP) induced by dibutyltin dichloride. Methods: Thirty healthy male Wistar rats were divided into the normal control group, CP group and CP + MXD-treated group. CP was induced by injection of dibutyitin dichloride (DBTC, 7 mg / kg of body weight) into the right caudal vein, and the control rats were treated with vehicle. MXD was given daily by gavage at a dose of 10 g / kg of body weight, starting from the day after CP induction. After 28-day treatment, the n-benzoyl- tyrosyl para-aminobenzoic acid (NBT-PABA) test was carried out to evaluate exocrine pancreatic function. Chen, rats were sacrificed, and pancreatic tissues were harvested for histological evaluation. In addition, the mRNA expression of TGF- type II receptor (TGFβRII), Smad3 and Smad7 was determined in pancreatic tissue s by using real-time polymerase chain reaction. Results: Treatment of CP with MXD improved the PABA recovery, decreased the histological lesion, and reduced the mRNA expression of TGF-β1, TGFβRII and Smad3 (P <0.05) .However, MXD had no effect on Smad7 mRNA level. Conclusions: MXD could protect the pancreas against chronic injury and improve pancreatic exocrine function in DBTC induced rat CP model. Its mechanism may involve inhibition of the TGF-β1 / Smads signaling pathway.