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目的了解系统性红斑狼疮(SLE)患者外周血CD4+CD2+5T细胞表达及其临床意义。方法用流式细胞仪检测53例SLE患者外周血CD4+CD2+5T细胞表达,根据CD25表达荧光强度>100者为CD4+CD25brightT细胞,并与SLE活动指数评分(SLEDAI)、血清补体C3水平、抗双链DNA抗体、抗核抗体滴度进行相关分析。结果SLE患者外周血CD4+CD2+5T细胞表达率为(7·84±1·85)%,显著低于对照组(9·18±2·01)%(P<0·05),且活动期组[(6·72±1·16)%]较稳定期组更低[(8·57±1·91)%,P<0·01]。外周血CD4+CD25brightT细胞表达率在活动期组[(0·85±0·24)%]和稳定期组[(0·91±0·25)%]之间相比差异无统计学意义(P=0·686),但均低于对照组[(1·43±1·08)%,P<0·01]。随治疗后SLEDAI评分的下降,活动期组SLE患者外周血CD4+CD25brightT细胞表达率无明显变化。进一步分析外周血CD4+CD25brightT细胞表达率与SLEDAI评分、抗核抗体、抗双链DNA抗体滴度和血清C3水平的相关性,分别为ρ=-0·188,P=0·178;ρ=-0·216,P=0·121;ρ=0·082,P=0·560;ρ=0·010,P=0·944,差异均无统计学意义(P>0·05)。结论外周血CD4+CD2+5T细胞的减少可能与SLE的发病有关。
Objective To investigate the expression of CD4 + CD25 + T cells in peripheral blood of patients with systemic lupus erythematosus (SLE) and its clinical significance. Methods Flow cytometry was used to detect the expression of CD4 + CD25 + T cells in peripheral blood of 53 patients with SLE. According to the expression of CD25, CD4 + CD25brightT cells with fluorescence intensity> 100 were analyzed. The results were compared with those of SLE activity index (SLEDAI), serum complement C3, Anti-double-stranded DNA antibody, antinuclear antibody titer correlation analysis. Results The positive rate of CD4 + CD25 + T cells in peripheral blood of patients with SLE was (7.884 ± 1.85)%, which was significantly lower than that of the control group (9.18 ± 2.01)% (P <0.05) The difference was statistically significant (P <0.01), which was significantly lower than that in the stable group [(6.72 ± 1.16)% vs (8.77 ± 1.91)%, P <0.01). The positive rate of CD4 + CD25brightT cells in peripheral blood was no significant difference between active group [(0.85 ± 0.24)%] and stable group (0.91 ± 0.25%) ( P = 0.686), but both were lower than those in the control group (1.43 ± 1.08%, P <0.01). With the decline of SLEDAI score after treatment, the expression rate of CD4 + CD25brightT cells in peripheral blood of patients with active SLE had no significant change. Further analysis of peripheral blood CD4 + CD25brightT cell expression and SLEDAI score, antinuclear antibody, anti-double-stranded DNA antibody titers and serum C3 levels were related to ρ = -0 · 188, P = 0.178; ρ = -0.216, P = 0.121; ρ = 0.082, P = 0.560; ρ = 0.010, P = 0.944, the difference was not statistically significant (P> 0.05). Conclusion The decrease of CD4 + CD2 + 5T cells in peripheral blood may be related to the pathogenesis of SLE.