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目的:研究板蓝根活性提取物体外抗呼吸道合胞病毒(RSV)的作用。方法:MTT法检测人喉癌上皮细胞(hep-2)中板蓝根活性提取物对RSV的抑制作用。荧光定量RT-PCR检测药物对RSV非结构蛋白NS1和L蛋白RNA合成的影响。结果:MTT结果显示药物治疗组和预防组在0.25~1 mg/m L浓度时均表现出抑制RSV的作用,其中0.5 mg/m L药物对RSV的抑制率分别是34.2%和52.2%,药物没有显示出直接杀伤和抑制RSV的效应。荧光定量RT-PCR检测表明药物作用1 h,RSV的NS1和L的RNA水平均显著低于病毒组(P<0.05)。结论:RSV感染hep-2过程中,板蓝根活性提取物具有预防和早期抗病毒复制的效应,但未显示出直接杀伤RSV的功能。
Objective: To study the anti-respiratory syncytial virus (RSV) activity of Banlangen active extract in vitro. Methods: MTT assay was used to detect the inhibitory effect of extract of Radix isatidis in human laryngeal carcinoma epithelial cells (hep-2) on RSV. Fluorescent quantitative RT-PCR was used to detect the effect of drugs on RNA synthesis of RSV nonstructural proteins NS1 and L proteins. Results: The results of MTT showed that RSV was inhibited in the drug-treated group and the prophylaxis group at a concentration of 0.25-1 mg / m L, of which the inhibition rate of RSV by 0.5 mg / m L was 34.2% and 52.2%, respectively The effects of direct killing and inhibition of RSV were not shown. Fluorescent quantitative RT-PCR assay showed that the RNA level of NS1 and L of RSV was significantly lower than that of the virus group (P <0.05) after 1 h of drug treatment. CONCLUSION: The active extract of Radix Isatidis has the effect of preventing and early antiviral replication during hep-2 RSV infection, but it does not show the function of directly killing RSV.