对昆明鼠自发性纤维肉瘤细胞进行体外培养并建立昆明鼠纤维肉瘤细胞系（ＫＭＦ９２）至１９９２年１２月已传８９代群体倍增时间９６ｈ，集落形成率２６７％，染色体众数７９～８１条，细胞异型性明显，成束或漩涡状排列胞质内未见肌微丝，胞质粗面内质网甚多，外质膜未见外板ＫＭ，ＢＡＬｂ／ｃ鼠移植生长瘤结，并保持原发瘤生物特征ＫＭＦ９２的建立，有利于纤维肉瘤细胞生物学的研究及抗癌药筛选，并可进一步研究昆明鼠的生物学特性 Kunming mouse fibrosarcoma cells were cultured in vitro and a Kunming mouse fibrosarcoma cell line (KMF92) was established. By December 1992, the doubling time of the population was 89 hours and the colony formation rate was 267%. Numbers 79-81, cell atypicality was obvious, no myofilament was seen in the cytoplasm of bundles or whirlpools, there were many cytoplasmic rough endoplasmic reticulums, and there was no outer plate in outer plasma membrane KM, BALb/c The transplantation of tumor growth in mice and the establishment of KMF92 primary tumor biological characteristics are conducive to the study of fibrosarcoma cell biology and anticancer drug screening, and can further study the biological characteristics of Kunming mice.