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乳腺癌干细胞(BCSCs)的主要分选方法包括利用肿瘤干细胞的表面分子标记物及肿瘤干细胞的功能特性进行分选,培养方法主要为无血清悬浮培养法。目前BCSCs的分选方法和培养方法尚不能满足BCSCs研究的需要。本文就BCSCs的各种分选和培养方法作一综述。
The main sorting methods for breast cancer stem cells (BCSCs) include the use of surface markers of cancer stem cells and the functional characteristics of tumor stem cells. The main methods of culture are serum-free suspension culture. Currently BCSCs sorting methods and culture methods can not meet the needs of BCSCs research. This article reviews various methods of sorting and culturing BCSCs.