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目的:探讨促性腺激素释放激素(GnRH)对人鼻咽癌CNE2细胞活力、凋亡、迁移和侵袭能力的影响。方法:收集鼻咽癌和慢性鼻咽炎症的组织标本,采用免疫组织化学染色、qRT-PCR分析检测标本中GnRH阳性细胞、GnRH mRNA的表达。体外培养人鼻咽癌CNE2细胞,永生化鼻咽上皮细胞株NP69,采用免疫组织化学染色,qRT-PCR分析检测细胞中GnRH阳性细胞、GnRH mRNA的表达。以不同浓度0(空白对照组)、10~(-2)、10~(-1)、10~0nmol/L GnRH处理CNE2细胞,采用CCK-8、流式细胞术、划痕修复实验、Transwell小室体外侵袭实验观察GnRH对CNE2细胞活力、凋亡、迁移和侵袭能力的影响。结果:鼻咽癌组织中GnRH阳性细胞、GnRH mRNA表达明显低于慢性鼻咽炎症组织(P<0.05)。CNE2细胞中GnRH阳性细胞、GnRH mRNA表达明显低于NP69(P<0.05)。与空白对照组比较,GnRH显著抑制CNE2细胞活力、凋亡、迁移和侵袭能力(P<0.05或P<0.01)。结论:GnRH显著抑制CNE2细胞活力、凋亡、迁移和侵袭能力。
Objective: To investigate the effect of gonadotropin-releasing hormone (GnRH) on the viability, apoptosis, migration and invasion of human nasopharyngeal carcinoma CNE2 cells. Methods: Tissue samples of nasopharyngeal carcinoma and chronic nasopharyngitis were collected. The expression of GnRH positive cells and GnRH mRNA were detected by qRT-PCR and immunohistochemical staining. Human nasopharyngeal carcinoma CNE2 cells and immortalized nasopharyngeal epithelial cell line NP69 were cultured in vitro, and the expression of GnRH positive cells and GnRH mRNA were detected by qRT-PCR and immunohistochemical staining. CNE2 cells were treated with GnRH at different concentrations (0, 10 ~ (-2), 10 ~ 10 nmol / L for 10 days, CCK-8, The cell invasion assay in vitro was used to observe the effect of GnRH on the viability, apoptosis, migration and invasion of CNE2 cells. Results: The expression of GnRH positive cells and GnRH mRNA in nasopharyngeal carcinoma tissues was significantly lower than that in chronic nasopharyngeal inflammatory tissues (P <0.05). The expression of GnRH mRNA in GnRH positive cells in CNE2 cells was significantly lower than that in NP69 cells (P <0.05). Compared with the blank control group, GnRH significantly inhibited the viability, apoptosis, migration and invasion ability of CNE2 cells (P <0.05 or P <0.01). Conclusion: GnRH significantly inhibits the viability, apoptosis, migration and invasion of CNE2 cells.