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目的:以甘肃贝母鳞茎为外植体,建立组培快繁体系,为资源保护和有效利用奠定基础。方法:研究了不同的外植体消毒方法,不同的激素浓度及配比,不同的培养条件等对原球茎发生、生长膨大、发芽生长的影响。结果:通过内吸性杀菌剂预处理,结合二次消毒可以较有效的控制外植体的污染;原球茎诱导过程中以鳞瓣为外植体的最优激素组合是ZT 3 mg/L+MEJA 3 mg/L+IAA 3 mg/L+BR 0.3 mg/L(或GA 4+7 0.2 mg/L),以鳞心为外植体最优激素组合是ZT 2 mg/L+SA 10 mg/L+BR 0.2 mg/L(或GA 4+7 0.2 mg/L),鳞心诱导效率高于鳞瓣;原球茎生长膨大阶段,鳞瓣原球茎生长膨大的最优激素组合是NAA 3 mg/L+SA 40 mg/L+BR 0.3 mg/L(或S-3307 5mg/L),鳞心原球茎生长膨大的最优激素组合是NAA 2 mg/L+SA 20 mg/L+BR 0.3 mg/L(或S-3307 3 mg/L),鳞瓣原球茎生长膨大效果明显优于鳞心原球茎;不同大小的试管小鳞茎在发芽生长特性方面存在较大差异。结论:建立了甘肃贝母试管小鳞茎的组培再生体系。
OBJECTIVE: To establish a rapid propagation system for tissue culture with bulbs of Fritillaria indica Gansu as the explant, laying the foundation for resource conservation and effective utilization. Methods: The effects of different methods of disinfection of explants, different hormone concentration and ratio, different culture conditions on protocorm growth, growth and germination were studied. Results: The systemic disinfectant pretreatment combined with secondary disinfection could effectively control the contamination of explants. The optimal hormone combination with scale flap as explant in the process of protocorm induction was ZT 3 mg / L + MEJA 3 mg / L + IAA 3 mg / L + BR 0.3 mg / L (or GA 4 + 7 0.2 mg / L). The optimal hormone combination was ZT 2 mg / L + SA 10 mg / L + BR 0.2 mg / L (or GA 4 +7 0.2 mg / L), and the center of the scale was more efficient than the scale flap. The optimal hormone combination of protocorm bulge growth in protocorm growth was NAA 3 mg The optimal hormone combination of bulblets for bulblets was NAA 2 mg / L + SA 20 mg / L + BR 0.3 / L + SA 40 mg / L + BR 0.3 mg / L or S-3307 5 mg / (or S-3307 3 mg / L). The growth of protocorm-like bodies in scales was significantly better than that of protocorm-like bodies. Bulblets in different sizes showed significant differences in germination and growth characteristics. Conclusion: The tissue culture regeneration system of the small bulb in vitro of Fritillaria thunbergii was established.