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目的观察肺表面活性蛋白A、D(SP-A、SP-D)在肺炎患儿支气管肺泡灌洗液(BALF)中的表达,探讨SP-A、SP-D与肺炎患儿临床特征的相关性。方法 35例肺炎患儿纳入研究,对其肺泡灌洗液细胞进行分类计数,采用酶联免疫吸附试验(ELISA)检测患儿BALF中SP-A和SP-D水平。结果患儿BALF中SP-D水平显著高于SP-A水平(P<0.001)。BALF中SP-D水平与中性粒细胞百分比成负相关(rs=-0.5255,P<0.01);血C反应蛋白水平升高(>8 mg/L)的患儿BALF中SP-D水平明显低于C反应蛋白正常的患儿(P<0.05);有喘息症状的患儿BALF中SP-D水平明显低于无喘息症状者(P<0.01)。BALF中的SP-A与临床特征无明显关系(P>0.05)。结论肺炎患儿BALF中SP-D的表达显著高于SP-A,与肺炎患儿的临床特征有一定相关性,作为一种保护性因子在调节免疫及炎症反应中发挥着比SP-A更重要的作用。
Objective To observe the expression of SP-A and SP-D in bronchoalveolar lavage fluid (BALF) of children with pneumonia and to investigate the correlation between the clinical features of SP-A and SP-D in children with pneumonia Sex. Methods 35 cases of children with pneumonia were enrolled in the study. The alveolar lavage fluid cells were classified and counted. The levels of SP-A and SP-D in BALF were detected by enzyme linked immunosorbent assay (ELISA). Results The level of SP-D in BALF of children was significantly higher than that of SP-A (P <0.001). The level of SP-D in BALF was negatively correlated with the percentage of neutrophils (rs = -0.5255, P <0.01). The level of SP-D in BALF was significantly higher in children with elevated serum C-reactive protein (> 8 mg / L) (P <0.05). The level of SP-D in BALF was significantly lower in children with wheeze than in those without wheeze (P <0.01). There was no significant correlation between SP-A and clinical features in BALF (P> 0.05). Conclusions The expression of SP-D in BALF of children with pneumonia is significantly higher than that of SP-A, which is correlated with the clinical features of children with pneumonia. As a protective factor, the expression of SP-D in BALF plays a more important role in regulating immune and inflammatory reactions than SP-A Important role.