论文部分内容阅读
目的对山东德州黄花败酱超临界萃取物进行分析鉴定,测定其抑菌活性及最小抑菌浓度(MIC)。方法采用超临界CO2萃取法(SFE-CO2)提取黄花败酱的有效成分,用气相色谱-质谱(GC-MS)对其萃取物进行分离鉴定,采用琼脂扩散法测定萃取物的抑菌活性,采用微量稀释法测定其最小抑菌浓度(MIC)。结果共分离出了24种化学成分,黄花败酱超临界萃取物对沙门氏菌、福氏痢疾杆菌、金黄色葡萄球菌均有明显抑制作用,对大肠杆菌无明显抑制作用,其对福式痢疾杆菌、金黄色葡萄球菌的最小抑菌浓度为4 mg·mL-1,对沙门氏菌的最小抑菌浓度为5 mg·mL-1。结论为败酱属植物资源的进一步开发应用提供科学而确切的实验依据。
OBJECTIVE To analyze and identify the supercritical fluid extract of Dendrolimus punctatus in Dezhou, Shandong Province, and to determine the antibacterial activity and the minimum inhibitory concentration (MIC). Methods SFE-CO2 was used to extract the active constituents of Paeonia lactiflora. The extracts were isolated and identified by gas chromatography-mass spectrometry (GC-MS). The antibacterial activities of the extracts were determined by agar diffusion method. The minimum inhibitory concentration (MIC) was determined by microdilution method. Results A total of 24 chemical constituents were isolated. The supercritical fluid extract of Rhizoma Coptidis had obvious inhibitory effect on Salmonella, Shigella flexneri and Staphylococcus aureus, but had no inhibitory effect on Escherichia coli. Staphylococcus aureus minimum inhibitory concentration of 4 mg · mL-1, the minimum inhibitory concentration of Salmonella 5 mg · mL-1. The conclusion provides a scientific and exact experimental basis for the further development and application of Patrinia resources.