目的：选用合适的质粒表达载体，以多拷贝基因的形式在大肠杆菌中高效表达人α心钠素，为人α心钠素的下游纯化工作及基因工程菌的中试打下基础。方法：采用ＰＣＲ，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。结果：ＰＣＲ方法扩增αｈＡＮＰ基因，克隆至原核表达载体ｐＭＳ３１ｂ，热诱导表达融合蛋白。结论：在大肠杆菌中获得了人α心钠素的高表达。 OBJECTIVE: To select the appropriate plasmid expression vector and express human α-ANA in E.coli in the form of multi-copy gene, laying a foundation for the downstream purification of human α-atrial natriuretic peptide and the pilot-scale of genetically engineered bacteria. Methods: PCR, cloning and continuous subcloning, sequence analysis, prokaryotic temperature induced expression and other methods. Results: The αhANP gene was amplified by PCR and cloned into prokaryotic expression vector pMS31b. The fusion protein was induced by heat. Conclusion: High expression of human alpha atrial natriuretic peptide is obtained in E. coli.