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目的回顾分析26例白血病和实体瘤患儿外周血干细胞采集效果及其对受体造血恢复影响,筛选可能与采集效率提高和受体造血恢复相关的因素。方法总结26例供体的49次粒系集落刺激因子(G-CSF)动员后的外周血采集过程,统计分析供体动员剂量、采集时外周血白细胞、收获的单个核细胞(MNC)和CD34+细胞数、受体造血恢复三者间的相关性。结果26例供体均能很好耐受动员和采集过程。采集物MNC和CD34+细胞中位数分别为4.5×108/kg和1.9×106/kg。动员剂量与采集物中MNC数呈正相关,与采集物CD34+细胞数无相关。采集当天供体外周血白细胞总数对采集细胞量无影响,而MNC水平与CD34+细胞采量正相关。结论受体的造血恢复与采集物中的CD34+细胞数可能存在相关性。因此这类患儿的采集时有必要动态监测其动员时的外周血CD34+细胞水平,确定最佳采集时间,以获取足够的CD34+细胞采量。
Objective To retrospectively analyze the effect of collecting peripheral blood stem cells in 26 children with leukemia and solid tumor and their effects on the hematopoietic recovery of the recipients. Screening may be related to the factors related to the increase of collection efficiency and the recovery of hematopoietic receptor. Methods The peripheral blood collected from 26 donor donors after G-CSF mobilization was collected. The donor mobilization dose, peripheral blood leukocytes, harvested mononuclear cells (MNCs) and CD34 + Cell number, receptor hematopoiesis recovery correlation between the three. Results All 26 donors were well tolerated mobilization and collection process. The median numbers of collected MNC and CD34 + cells were 4.5 × 108 / kg and 1.9 × 106 / kg, respectively. The mobilization dose was positively correlated with the number of MNC in the collection, but not with the number of CD34 + cells collected. The total number of peripheral blood leukocytes on the day of collection had no effect on the amount of collected cells, while the level of MNC was positively correlated with the amount of CD34 + cells collected. Conclusions There may be a correlation between the recovery of hematopoietic recipients and the number of CD34 + cells in the collected samples. Therefore, the collection of these children need to dynamically monitor the mobilization of peripheral blood CD34 + cell levels, to determine the best acquisition time, in order to obtain sufficient CD34 + cell acquisition.