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目的 探讨蛋白酶ICH 1在原发性肝细胞性肝癌 (HCC)发生中的作用及与乙型肝炎病毒 (HBV)和丙型肝炎病毒 (HCV)感染的关系。方法 应用免疫组织化学方法对 40例原发性肝癌和癌旁组织中蛋白酶ICH 1 (ICH 1L和ICH 1S)、HBsAg及HCV非结构区 5抗原 (NS5抗原 )的表达进行观察。结果 40例肝癌中 1 5例ICH 1L阳性 ,阳性率为 37.5 % ,2 1例ICH 1S阳性 ,阳性率为 52 .5 %。癌细胞ICH 1表达较正常肝细胞增加 ,而又明显低于癌旁肝细胞 (P <0 .0 5)。 2 3例HBsAg和NS5阳性病例中 (其中两者共同阳性 3例 ) ,ICH 1L阳性 1 0例 ,占ICH 1L阳性总例数的 66 .7% (1 0 / 1 5) ,ICH 1S阳性 1 3例 ,占ICH 1S阳性总例数的 61 .9%。结论 蛋白酶ICH 1表达异常与肝细胞癌变有关 ,在肝癌发生过程中可能起一定作用。HBV和HCV感染可能是引起ICH 1表达异常的两种较重要的病因。
Objective To investigate the role of protease ICH 1 in the development of primary hepatocellular carcinoma (HCC) and its relationship with hepatitis B virus (HBV) and hepatitis C virus (HCV) infection. Methods The expression of protease ICH 1 (ICH 1L and ICH 1S), HBsAg and HCV non-structural domain 5 antigen (NS5 antigen) in 40 primary hepatocellular carcinoma tissues and adjacent tissues were observed by immunohistochemistry. Results Among the 40 cases of hepatocellular carcinoma, ICH 1L was positive in 15 cases, the positive rate was 37.5%, and 21 cases were positive for ICH 1S. The positive rate was 52.5%. The expression of ICH 1 in cancer cells was higher than that in normal liver cells, but significantly lower than that in para-cancerous hepatocytes (P <0.05). Among 23 cases of HBsAg and NS5 positive cases, of which 3 cases were positive, ICH 1L was positive in 10 cases, accounting for 66.7% (100/1) of ICH 1L positive cases and ICH 1S positive 1 3 cases, accounting for 61.9% of the total number of ICH 1S positive cases. Conclusion The abnormal expression of protease ICH 1 is related to the carcinogenesis of hepatocellular carcinoma and may play a role in the carcinogenesis of hepatocellular carcinoma. HBV and HCV infection may be the two most important causes of ICH1 expression abnormalities.