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目的检测Fas、促血管生成素-1(Ang-1)、促血管生成素-2(Ang-2)、E-钙粘蛋白(E-cad)在卵巢上皮肿瘤组织中的表达水平,藉此评价其与肿瘤发生发展及预后的关系。方法应用免疫组织化学S-P法和原位杂交技术,检查了21例卵巢上皮恶性肿瘤,18例良性肿瘤,8例正常卵巢组织中Fas,Ang-1,Ang-2及E-cad的表达。结果 Fas的表达随肿瘤病变程度的增高而逐步减弱,恶性肿瘤组织与正常卵巢及良性肿瘤组织间有显著差异(P<0．05)；E-cad免疫反应阳性强度在卵巢上皮癌组织中明显降低,与正常卵巢及良性肿瘤组织间有显著性差异(P<0．05)。原位杂交显示,Ang-1 mRNA在正常卵巢及肿瘤组织中都有表达,其表达水平没有显著差异(P>0．05)；Ang-2 mRNA在恶性肿瘤组织中表达明显上调(P<0．01)；E-cad mRNA在恶性肿瘤组织中表达明显下调(P<0.01)。结论 Fas的表达水平与卵巢上皮肿瘤的内在发展相关；由E-cad介导的细胞间黏附作用的减弱是卵巢癌发生、发展及转移的一个重要因素；Ang-2对于促进卵巢癌组织血管新生和形成可能具有重要促进作用。
Objective To detect the expression of Fas, Ang-1, Ang-2 and E-cad in epithelial ovarian tumor tissues Evaluate its relationship with tumorigenesis and prognosis. Methods The expressions of Fas, Ang-1, Ang-2 and E-cad in 21 cases of epithelial ovarian cancer, 18 cases of benign tumor and 8 cases of normal ovarian tissue were examined by immunohistochemical S-P method and in situ hybridization. Results The expression of Fas was gradually decreased with the increase of the degree of tumor. There was significant difference between the malignant tumor tissues and the normal ovary and the benign tumor tissues (P <0.05). The positive expression of E-cad in the ovarian epithelial carcinoma was significantly Decreased, and normal ovary and benign tumor tissue were significantly different (P <0.05). In situ hybridization showed that Ang-1 mRNA was expressed in normal ovary and tumor tissues, and the expression level of Ang-1 mRNA was not significantly different (P> 0.05). The expression of Ang-2 mRNA was significantly up-regulated in malignant tumor tissues .01). E-cad mRNA expression was significantly down-regulated in malignant tumors (P <0.01). Conclusion The expression level of Fas is correlated with the intrinsic development of ovarian epithelial neoplasm. The decrease of E-cadher cell adhesion is an important factor in the occurrence, development and metastasis of ovarian cancer. The effect of Ang-2 on promoting angiogenesis of ovarian cancer And formation may have an important facilitating role.