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目的 观察臂旁核 (PBN)内向丘脑腹后内侧核 (VPM)投射神经元和三叉神经脊束核尾侧亚核 (Vc)向臂旁核投射纤维和终末的分布 ,以及两者之间的突触联系。 方法 HRP逆行追踪与生物素葡聚糖胺 (BDA)顺行追踪相结合的双标技术 ,标记结果分别在光镜或电镜下观察。 结果 将 HRP注入 VPM后 ,在光镜下可见HRP逆标神经元主要位于同侧的臂旁外侧核、Kolliker- Fuse(KF)核和臂旁内侧核。将 BDA注入 Vc后 ,BDA顺标纤维和终末也主要见于同侧臂旁外侧核、KF核和臂旁内侧核 ,对侧丘脑 VPM、丘脑后核和丘脑胶状质核也有BDA顺标纤维和终末 ,但臂旁核内的 BDA顺标纤维和终末明显多于丘脑诸核团。在臂旁核内 ,HRP逆标神经元的主要分布区与 BDA顺标纤维和终末的主要分布区重叠。电镜下在臂旁核内可见 ,Vc向臂旁核投射的 BDA顺标纤维和终末与臂旁核向丘脑 VPM投射的 HRP逆标神经元形成以非对称性为主的轴 -树和轴 -体突触。 结论 大鼠中枢内存在 Vc- PBN- VPM间接联系通路 ,该通路在面口部躯体感觉信息 (包括伤害性信息 )的传递和调控过程中可能发挥重要作用。
OBJECTIVE: To observe the distribution of end-to-paracular nucleus (PMN) projection fibers in the medullary paraventricular nucleus (VPN) projecting neurons and the trigeminal subnucleus caudatum (Vc) of the paraventricular nucleus (PBN) Synaptic connection. Methods HRP retrograde tracing combined with biotin dextran amine (BDA) sequential tracing technique was used. The labeling results were observed under light or electron microscope respectively. Results HRP injection into the VPM showed that the HRP-labeled neurons were mainly located on the ipsilateral parabrachial lateral nucleus, Kolliker-Fuse (KF) nucleus and medial parabrachial nucleus. BDA injection of Vc, BDA cisplasma fibers and terminals are also mainly seen in the ipsilateral parabrachial nucleus, KF nucleus and parabrachial medial nuclei, contralateral thalamic VPM, thalamic nucleus and thalamic colloid also BDA cisplatin And terminal, but the parathyroid nucleus within the BDA-labeled fibers and terminals were significantly more than the thalamic nuclei. In the parabrachial nucleus, the major distribution of HRP retrograde neurons overlaps with the major distribution of BDA-labeled fibers and terminals. Electron microscope shows in the parabrachial nuclei that the BDA-labeled fibers projected by the Vc to the parabrachial nucleus and the HRP retrograde neurons terminating at the parabrachial nucleus to the VPM of the thalamus form an asymmetric predominantly axial-tree axis Synapse. Conclusion There is a Vc-PBN-VPM indirect pathway in the rat’s central nervous system. This pathway may play an important role in the transmission and regulation of somatosensory information, including nociceptive information.