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从构建的早园竹(Phyllostachys propinqua McClure)叶绿体DNA基因组文库中克隆了早园竹叶绿体atpA(EU64861)、psaB(EU64863)、rpoA(EU64862)和rpoC1(EU64864)4个基因,并将其序列分别与GenBank中部分禾本科植物的基因序列进行了比对分析,构建核苷酸序列的Neighbor-joining系统进化树,分析探讨其在禾本科中的分类地位。结果表明,禾本科植物在系统分类上呈现出两大类群。第一大类中包括两个亚类,水稻与竹类植物聚为一亚类;玉米、高粱和甘蔗聚为另一亚类。另一大类为小麦、大麦、匍茎剪股颖和黑麦草。表明早园竹与水稻分类地位最为接近;比水稻和玉米、高粱与甘蔗更为较近,与禾本科中麦类及其近缘植物关系较远。
Four genes of atpA (EU64861), psaB (EU64863), rpoA (EU64862) and rpoC1 (EU64864) were cloned from the chloroplast DNA library of Phyllostachys propinqua McClure. We compared the gene sequences of some gramineous plants in GenBank with the Neighbor-joining phylogenetic tree of nucleotide sequences and analyzed their taxonomic status in gramineae. The results showed that gramineous plants showed two major groups in the taxonomy. The first broad category includes two sub-categories, rice and bamboo plants clustered into a sub-category; corn, sorghum and sugar cane clustered into another sub-category. The other major category is wheat, barley, stolonifer and ryegrass. The results showed that the early stage bamboo and rice had the closest taxonomic status than the rice and maize, the sorghum and the sugarcane, and had a close relationship with the wheat and its related plants in the grass family.