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目的 研究树突状细胞（ Ｄ Ｃ）体外诱导的细胞免疫能否抑制裸鼠移植瘤肿瘤细胞增殖。方法 联合应用粒／巨细胞集落刺激因子（ Ｇ Ｍ Ｃ Ｓ Ｆ）及白介素４（ Ｉ Ｌ４）直接从肝癌患者外周血中培养出 Ｄ Ｃ；以源于人肝癌细胞系 Ｈｅｐ Ｇ２ 细胞的肿瘤抗原粗提物刺激 Ｄ Ｃ； Ｄ Ｃ 激活同源的 Ｔ 淋巴细胞产生细胞 毒性 Ｔ 淋 巴细胞 （ Ｃ Ｔ Ｌ）； 建立 人肝癌 细胞 系 Ｈｅｐ Ｇ２ 裸鼠移植瘤模型； Ｃ Ｔ Ｌ 治疗人肝癌细胞系 Ｈｅｐ Ｇ２ 裸鼠移植瘤；检测移植瘤标本肿瘤细胞 Ｐ Ｃ Ｎ Ａ 表达水平。结果 Ｄ Ｃ诱导的 Ｃ Ｔ Ｌ抑制 Ｈｅｐ Ｇ２ 裸鼠移植瘤细胞增殖从而抑制移植瘤生长。结论 经肿瘤抗原激活的 Ｄ Ｃ 作为一新概念上的抗肿瘤疫苗有可能在肿瘤的治疗中发挥重要作用。
Objective To investigate whether the cellular immunity induced by dendritic cells (DC) in vitro can inhibit the proliferation of tumor cells in nude mice. Methods D C was cultured directly from the peripheral blood of patients with hepatocellular carcinoma in combination with granulocyte/macrophage colony stimulating factor (G M C S F) and interleukin 4 (IL-4); it was derived from human hepatocellular carcinoma cell line Hep G2 cells. The crude extract of tumor antigen stimulates D C; D C activates homologous T lymphocytes to produce cytotoxic T lymphocytes (C T L); Hepatoma cell line Hep G2 is transplanted in nude mice; C T L is used to treat human liver cancer Hep G2 tumor xenografts in nude mice were used to detect the expression of P C N A in tumor cells. Results The DTL induced CTL inhibited the growth of Hep G2 transplanted tumor cells in nude mice and inhibited the growth of xenografts. Conclusion The tumor-activated D C as a new conceptual anti-tumor vaccine may play an important role in the treatment of tumors.