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目的建立改良抗体结合试验(Modified Antibody Binding Test,MABT)的检测流程,并试用于人用狂犬病疫苗(Rabies Vaccine for Human Use,RabV)的效价检测。方法由省级疾病预防控制中心(Center for Disease Control and Prevention,CDC)提供RabV样品,采用制订的MABT流程测定RabV样品效价。结果 MABT检测可以显示RabV样品效价的相对高低,A和B两个RabV样品经MABT测定效价均为4.4国际单位(International Unit,IU)/毫升(ml)(>2.5IU/ml)。结论制订的MABT检测流程工作稳定,MABT可以用于RabV效价的辅助检测。
Objective To establish a modified Antibody Binding Test (MABT) detection protocol and to test its potency against Rabies Vaccine for Human Use (RabV). Methods RabV samples were provided by the Provincial Center for Disease Control and Prevention (CDC) and the RabV sample titer was determined using the established MABT protocol. Results The MABT assay showed the relative titer of RabV samples. Both RabV samples of A and B had an International Organism Unit (IU) / ml (> 2.5IU / ml) as determined by MABT. Conclusions The MABT testing procedure is stable and MABT can be used to detect the RabV titer.