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为了验证5个抗旱主效QTL的导入效果,将已精细定位的5个抗旱主效QTLs(DTY1.1,DTY2.1,DTY2.2,DTY3.1和DTY12.1)作供体建立近等基因系,利用与5个QTL位点紧密连锁的16个SSR分子标记对导入抗旱主效基因的23份家系进行目标QTL检测。结果显示,利用6个标记同时检测,获得携带DTY3.1 QTL的家系5株;利用2个标记同时检测,获得携带DTY12.1 QTL的家系5株;其他3个QTL位点未检测到目标QTL的家系;没有同时获得具备不同抗旱QTL的家系。相比较而言,携带DTY3.1 QTL以Apo为供体及携带DTY12.1 QTL以Way Rarem为供体导入效果更好。这些抗旱家系的获得,为抗旱品种的选育提供了新的抗旱基因来源。
In order to verify the introduction effect of QTLs for 5 major drought-resistance traits, five QTLs (DTY1.1, DTY2.1, DTY2.2, DTY3.1 and DTY12.1) Gene lines. Twenty-six SSR markers closely linked to five QTLs were used to detect the target QTLs in 23 pedigrees introduced with drought resistance major genes. The results showed that 5 families of DTY3.1 QTLs were obtained by simultaneous detection of 6 markers; 5 families of DTY12.1 QTLs were obtained by simultaneous detection of 2 markers; no target QTLs were detected in the other 3 QTLs ; No pedigree with different QTLs for drought resistance was obtained at the same time. In contrast, carrying the QTL for DTY3.1 with Apo as a donor and carrying the QTL with DTY12.1 as a donor with Way Rarem performed better. The availability of these drought-resistant pedigrees provides a new source of drought-resistance genes for breeding drought-resistant varieties.