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目的探讨褪黑素(MT)对脂多糖(LPS)致脑损伤幼鼠脑组织胶质纤维酸性蛋白(GFAP)表达的影响及意义。方法160只1月龄SD大鼠随机分为LPS组、9g/L盐水组(NS组)、MT保护A组(MT加LPS,提前15min予MT)及MT保护B组(MT加LPS,即时给予MT)4组,每组40只。各组均按观察时间分为24、48h2个时间点,每组各时间点均为20只,其中均有10只不注射伊文思蓝(EB),观察至相应时间点取其脑组织。以甲酰胺法检测其脑组织EB水平,免疫组织化学染色法检测其GFAP表达,采用上海山富数字图像分析系统进行半定量分析。结果LPS组脑组织EB水平、GFAP阳性区面积与NS组各相应时间点比较差异均有统计学意义(Pa<0.01);MT保护组EB水平、GFAP阳性区面积均显著高于NS组(Pa<0.01);MT保护组EB水平、GFAP阳性区面积均显著低于LPS组(Pa<0.01);MT保护组组间比较无显著性差异(P>0.05)。结论LPS可诱导内毒素致大鼠感染性脑损伤GFAP的表达;MT可能在治疗感染性脑损伤中发挥作用。
Objective To investigate the effect and significance of melatonin (MT) on glial fibrillary acidic protein (GFAP) expression in brain of immature rats with lipopolysaccharide (LPS) -induced brain injury. Methods One hundred and sixty one-month-old Sprague-Dawley rats were randomly divided into three groups: LPS group, NS group (NS group), MT group (MT plus LPS group, MT group) Given MT) 4 groups of 40. Each group was divided into observation time 24,48h2 time points, each time point was 20, of which 10 were not injected Evans blue (EB) observed at the appropriate time point to take the brain tissue. The EB level of brain tissue was detected by formamide method. The GFAP expression was detected by immunohistochemical staining and semi-quantitative analysis was performed by Shanghai Shanfu digital image analysis system. Results The levels of EB and GFAP in brain tissue of LPS group were significantly different from those of NS group at each time point (P <0.01). The levels of EB and GFAP in MT group were significantly higher than those in NS group <0.01). The level of EB and the area of positive GFAP in MT group were significantly lower than those in LPS group (P <0.01). There was no significant difference between MT group and control group (P> 0.05). Conclusion LPS can induce endotoxin-induced GFAP expression in rats with traumatic brain injury. MT may play a role in the treatment of infectious brain injury.