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目的 研究雄激素水平对良性前列腺增生 (BPH)组织中凋亡抑制基因Bcl 2mRNA表达的影响。 方法 分别通过服用药物和切除睾丸改变BPH患者和大鼠机体雄激素水平 ,采用mRNA斑点杂交技术 ,检测不同雄激素水平下的人BPH组织和大鼠前列腺组织Bcl 2mRNA的表达情况。 结果 在所有 2 0例人BPH组织中 ,Bcl 2mRNA表达均呈阳性 ,未服药对照组Bcl 2mRNA杂交斑点积分光密度值 (IOD值 )是服药组的 1 71倍 (P <0 0 1) ;正常对照组大鼠前列腺组织Bcl 2mRNA杂交斑点IOD值是去势组的 1 2 8倍 (P <0 0 5 )。 结论 抗凋亡基因Bcl 2的表达与BPH的发生有关 ,前列腺细胞凋亡的减少可能是BPH发病的重要原因之一。雄激素在BPH的发病中除了促进前列腺上皮和间质的增殖外 ,也可能通过促进Bcl 2的表达 ,抑制细胞凋亡而发挥作用
Objective To investigate the effect of androgens on the expression of Bcl-2 mRNA in benign prostatic hyperplasia (BPH). Methods The levels of serum androgen in BPH patients and rats were changed by taking drugs and excision of testis respectively. The expression of Bcl-2 mRNA in human BPH tissues and rat prostate tissues under different androgen levels was detected by mRNA dot blot hybridization. Results The Bcl 2 mRNA expression was positive in all 20 human BPH tissues. The integral optical density (IOD) value of Bcl 2 mRNA in non-medication control group was 171 times (P <0.01) The IOD value of Bcl 2 mRNA hybridization spots in the control group was 128 times that of the castrate group (P <0 05). Conclusions The expression of anti-apoptotic gene Bcl 2 is related to the pathogenesis of BPH. The decrease of prostatic cell apoptosis may be one of the important causes of BPH. Androgen in the pathogenesis of BPH in addition to promoting prostate epithelial and interstitial proliferation, but also may promote Bcl 2 expression, inhibit apoptosis and play a role