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目的研究南宁市某医院散发性急性胃肠炎病例中,GⅠ.8型诺如病毒(Norovirus,NV)的基因特征。方法采用荧光定量逆转录-聚合酶链反应,对2007年1月～2008年12月南宁市某医院散发性急性胃肠炎病例标本检测基因Ⅰ型(GenogroupⅠ,GⅠ)NV核糖核酸,对阳性标本进行N/S区核苷酸序列测定,并构建系统进化树分析。结果在696份粪便标本中,检出3份GⅠ型NV核酸阳性。经核酸序列分析发现,其中一份[南宁(Nanning,NN)07230]与GⅠ.8型参考株(WUGⅠ/00/JP)的核苷酸序列同源性达94.5%;与2006～2007年日本、韩国公布的GⅠ.8型NV核苷酸序列同源性范围在96.7%～98.5%。NN07230在进化树中与GⅠ.8型病毒株属同一分支。结论在南宁市散发性急性胃肠炎病例中存在GⅠ.8型NV感染。
Objective To investigate the genetic characteristics of Norovirus (GV) genotype I 1.8 in a sporadic acute gastroenteritis in a hospital in Nanning City. Methods Fluorescent quantitative reverse transcriptase polymerase chain reaction (RT - PCR) was used to detect Genogroup Ⅰ (G Ⅰ) NV RNA in sporadic acute gastroenteritis cases from a hospital in Nanning City from January 2007 to December 2008. The nucleotide sequence of N / S region was determined and phylogenetic tree analysis was constructed. Results Of the 696 stool specimens, three GVNV positive were detected. Nucleotide sequence analysis showed that the nucleotide sequence homology of one copy of [Nanning (NN) 07230] to GⅠ.8 type reference strain (WUGⅠ / 00 / JP) was 94.5% , Korea published G Ⅰ.8 NV nucleotide sequence homology in the range of 96.7% ~ 98.5%. NN07230 is in the same branch of the phylogenetic tree of type GI.8 in the phylogenetic tree. Conclusions G Ⅰ .8 NV infection exists in cases of sporadic acute gastroenteritis in Nanning.