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磷脂-蛋白相互作用的临界摩尔比是研究膜脂-蛋白相互作用的重要参数.本文利用荧光光谱技术首次测定了毒素蛋白ColicinE1在不同条件下与不同磷脂膜相互作用的临界摩尔比并通过临界摩尔比的变化讨论了插膜蛋白与磷脂膜相互作用的规律,为进一步探讨毒素蛋白的插膜机制提供了重要的基础
The critical molar ratio of phospholipid-protein interactions is an important parameter for studying membrane lipid-protein interactions. In this paper, the critical molar ratio of the toxin protein ColicinE1 to different phospholipid membranes was determined by fluorescence spectrometry for the first time and the interaction between the intercalator proteins and the phospholipid membrane was discussed by the change of the critical molar ratio. To further explore the interaction between the toxin protein The intercalation mechanism provides an important foundation