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目的：探讨ｃ－ｅｒｂＢ２反义核酸对卵巢癌细胞生物学行为及其化疗药物敏感性的影响。方法：应用ｐＵＣ－ｅｒｂＢ２和ｐＤＯＲ－ｎｅｏ质粒，构建了含反义ｅｒｂＢ２ｃＤＮＡ３．８ｋｂ的重组逆转录病毒表达载体ｐＤＯＲ－ｅｒｂＢ－ｎｅｏ，经脂质体转染ｅｒｂＢ２高表达的人卵巢癌细胞系ＳＫＯＶ３，再经Ｇ４１８（一种新霉素类似物）筛选得到新霉素抗性细胞，命名为ＳＫＯＶ３－Ａ２。结果：Ｓｏｕｔｈｅｒｎ印迹杂交分析表明，反义ｅｒｂＢ２３．８ｋｂ已完全整合到ＳＫＯＶ３细胞中，与亲本细胞及转染ｐＤＯＲ－ｎｅｏ的对照组细胞相比，ＳＫＯＶ３－Ａ２的生长和ＤＮＡ合成均受到抑制，且对５－氟尿嘧啶（５－ＦＵ）及顺铂（ＤＤＰ）的药物敏感性明显增加。结论：ｃ－ｅｒｂＢ２反义核酸在卵巢癌基因治疗中有一定作用；ｃ－ｅｒｂＢ２癌基因可能与卵巢癌细胞耐药有关。
Objective: To investigate the effect of c-erbB2 antisense oligonucleotide on the biological behavior and chemosensitivity of ovarian cancer cells. Methods: The recombinant retroviral vector pDOR-erbB-neo containing antisense erbB2 cDNA was constructed by using pUC-erbB2 and pDOR-neo plasmids. The human erbB2-overexpressed human ovarian cancer cell line SKOV3 was transfected with pDOR- Then neomycin resistant cells were screened by G418 (a neomycin analogue) and named as SKOV3-A2. Results: Southern blot analysis showed that antisense erbB23.8kb was completely integrated into SKOV3 cells, and the growth and DNA synthesis of SKOV3-A2 were both inhibited compared with parental cells and control cells transfected with pDOR-neo, and Drug sensitivity to 5-fluorouracil (5-FU) and cisplatin (DDP) was significantly increased. Conclusion: The c-erbB2 antisense nucleic acid plays a role in the gene therapy of ovarian cancer. The c-erbB2 oncogene may be related to the drug resistance of ovarian cancer cells.