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选择6只新西兰大耳白兔，在其每根股骨的中段制备两个直径3mm的骨缺损，植入1.5Xllmm的圆柱状钛钟植体，使种植体与原合之间形成一定间隙，在其中一个种植体表面覆盖钛膜，另一个不覆盖作为对照。并在种植区邻近肌肉内将钛膜常规埋植。分别于术后2，3，4周处死动物，作肉眼、光镜观察。结果表明：肌肉内钛膜植入区无炎性反应，其周围的纤维组织结构及细胞随时间增加而成熟术膜覆盖的种植体周围无来自种植窝外的纤维组织，而在无钛膜覆盖的种植体周围，种植窝外的纤维组织向间隙内生长；与对照部位的同期相比，实验部位的种植体周围的成骨反应较明显，在骨皮质表面及内侧的新骨生长较早、较快。说明钛膜有引导组织再生的作用。
Six New Zealand white rabbits were selected to prepare two bone defects of 3 mm in diameter in the middle of each femur. 1.5 × 11 mm cylindrical titanium clocks were implanted to make a certain gap between the implant and the original One of the implants was covered with a titanium film on the surface and the other one was not covered as a control. And in the planting area adjacent to the titanium implant film conventional implantation. Animals were sacrificed at 2, 3, and 4 weeks postoperatively for visual observation and light microscopy. The results showed that there was no inflammatory reaction in the implantation zone of intramuscular titanium membrane, and the surrounding fibrous tissue and cells increased with time. There was no fibrous tissue around the implant from the mature implant membrane. However, The growth of fibrous tissue around the implant was observed in the gap. Compared with the same period of the control site, the osteogenic reaction around the implant at the experimental site was more obvious. The new bone grew on the surface and inside of the cortical bone earlier, Faster. Explain the role of titanium membrane guide tissue regeneration.