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上海第二军医大学附属长海医院临床实验诊断科沈茜等,利用生物素标记pCB Ⅲ/35和pCB Ⅲ/51肠道病毒属特异性探针,对40例心肌病尸检心肌材料及5例心肌炎心内膜活检材料进行了原位杂交。 结果显示:12例(26.6％)心肌病损组织中检出肠道病毒RNA,其中5例(41.7％)与pCBⅢ/29(柯萨奇B:病毒型特异性探针)出现阳性杂交信号,表明存在COXB:特异性RNA。在对17例同时获得左右心室心肌组织的病毒RNA检出情况比较中发现,在6例检出肠道病毒RNA的病损心肌组织
Shanghai Second Military Medical University, Changhai Hospital Affiliated Changhai Hospital Clinical Laboratory Diagnosis Division Qian Qian, the use of biotin-labeled pCB Ⅲ / 35 and pCB Ⅲ / 51 enterovirus-specific probes, 40 cases of myocardial necrosis myocardial cell material and 5 cases of myocarditis Endocardial biopsy material was in situ hybridized. The results showed that in 12 cases (26.6%) of the diseased myocardium, enterovirus RNA was detected, of which 5 (41.7%) showed positive hybridization with pCBⅢ / 29 (Coxsackie B: virus type specific probe) Indicates the presence of COXB: specific RNA. In 17 cases of simultaneous acquisition of left and right ventricular myocardium virus RNA detected in the comparison found in 6 cases of intestinal virus RNA detected lesions in myocardial tissue