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[目的]研究尼美舒利(NIM)是否通过过氧化物酶增殖物激活受体γ(PPAR-γ)途径影响胃癌细胞体内生长。[方法]通过培养人胃癌SGC-7901细胞,建立裸鼠胃癌移植瘤模型。设立GW9662+NIM组、生理盐水组、GW9662组、NIM组共4组。3周后测量各组裸鼠移植瘤体积,并采用免疫组化法检测裸鼠移植瘤组织PPAR-γ表达。[结果]NIM组裸鼠移植瘤体积较生理盐水组明显小,而GW9662+NIM组较NIM组移植瘤体积大,各组之间差异有显著性(P<0.05)。GW9662+NIM组与NIM组相比,移植瘤组织的PPAR-γ蛋白表达降低。[结论]尼美舒利在体内能抑制胃癌细胞的生长,PPAR-γ通路可能在此过程中发挥重要作用。
[Objective] To investigate whether nimesulide (NIM) affects the growth of gastric cancer cells in vivo through peroxisome proliferator-activated receptor γ (PPAR-γ) pathway. [Method] The model of gastric cancer xenografts in nude mice was established by culturing human gastric cancer SGC-7901 cells. GW9662 + NIM group, normal saline group, GW9662 group and NIM group were established. Three weeks later, the xenograft tumor volume of each group was measured, and the expression of PPAR-γ in nude mice xenografts was detected by immunohistochemistry. [Results] The volume of tumor xenografts in nude mice in NIM group was significantly smaller than that in saline group, while the volume of tumor in GW9662 + NIM group was larger than that in NIM group (P <0.05). GW9662 + NIM group compared with the NIM group, the transplanted tumor tissue PPAR-γ protein expression decreased. [Conclusion] Nimesulide can inhibit the growth of gastric cancer cells in vivo. PPAR-γ pathway may play an important role in this process.