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以河南洛阳各大主要牡丹园栽培的牡丹典型根腐病病株为试材,采用组织分离法获得纯菌株,并对所得菌株进行致病性测定、形态学鉴定及rDNA-ITS序列分析,研究牡丹根腐病的病原菌种类。结果表明:分离获得的4个菌株在PDA培养基上均产生茂密、呈疏松棉絮状的菌丝及黄、红、紫等色素,大型分生孢子镰刀形或椭圆形,无色,多胞;小型分生孢子卵圆形至椭圆形,无色,单胞或双胞。致病性测定表明,4个菌株能侵染离体牡丹根尖使其变黑。在GenBank序列数据库中,F1~F4菌株的DNA序列与编号为HM214456.1和AB498917.1等的F.solani ITS区段DNA序列的同源性为99%~100%。据此,将河南洛阳牡丹根腐病的病原菌鉴定为F.solani。
The typical strains of peony root rot cultivated in major peony gardens in Luoyang of Henan Province were used as test materials, and the pure strains were obtained by tissue isolation method. The pathogenicity, morphology and rDNA-ITS sequence analysis of the obtained strains were also studied Peony root rot pathogens species. The results showed that all the four isolates produced dense, loose cottony mycelium and yellow, red and purple pigments on PDA medium. The large conidia were sickle - shaped or oval, colorless and multicellular. Conidia ovoid to elliptic, colorless, single or double. Pathogenicity tests showed that four strains could invade the root tip of detached peony to darken. In the GenBank database, the DNA sequences of F1 ~ F4 strains shared 99% -100% homology with the DNA sequences of F. solani ITS sequences numbered HM214456.1 and AB498917.1. Accordingly, the pathogen of peony root rot in Luoyang, Henan was identified as F. solani.