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以结球甘蓝鸡心２３、晨光５１号两个自交不亲和系种子的无菌苗的下胚轴为外植体材料，在附加１ｍｇ／ｌＢＡ、１ｍｇ／ｌＺＴ、０．０５ｍｇ／ｌＮＡＡ或２ｍｇ／ｌＺＴ、０．０５ｍｇ／ｌＮＡＡ的稍作修改的ＭＳ培养基上诱导不定芽发生，不定芽诱导频率分别为８６．４％、１００％和８６．３％、４０．６％。头孢霉素有抑制结球甘蓝下胚轴诱导不定芽的作用，羧苄青霉素则能保持原有的不定芽诱导频率。卡那霉素在诱导不定芽的过程中，有完全抑制其发生的作用，而在不定芽和幼苗生长阶段则有筛选作用。卡那霉素敏感性测定结果，１０ｍｇ／ｌ卡那霉素为适宜的选择浓度。以下胚轴的切段为受体，应用农杆菌介导法将豇豆胰蛋白酶抑制剂基因（ＣｐＴＩ）和新霉素磷酸转移酶基因（ＮＰＴＩＩ）重组质粒导入结球甘蓝，获得卡那霉素抗性再生植株
Hypocotyls of aseptic shoots of two self-incompatible lines seedlings of cabbage heart23 and Chenguang51 were used as explant materials, with additional 1mg / lBA, 1mg / lZT, 0.05mg / lNAA or 2mg Adventitious buds were induced on slightly modified MS medium supplemented with 10mg / l ZA, 0.05mg / l NAA. The induction frequency of adventitious buds was 86.4%, 100% and 86.3%, 40.6% respectively. Cephalosporins can inhibit the hypocotyl induced cabbage hypocotyls role, carbenicillin can maintain the original induction of adventitious buds. In the process of adventitious bud induction, kanamycin completely inhibits the occurrence of kanamycin, while it has the function of screening during the process of adventitious buds and seedling growth. Kanamycin sensitivity assay results, 10mg / l kanamycin is the appropriate choice of concentration. The cuttings of the following hypocotyls were used as the receptor. Agrobacterium tumefaciens-mediated method was used to introduce CpTI and NPTII recombinant plasmids into cabbage, Sexually-regenerated plants