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目的：研究黄曲霉毒素高危区谷胱甘肽转移酶M1(GSTM1)基因多态性与肝细胞癌的相关性，验证该指标作为肝细胞癌预报因子的可靠性。方法：用特异性引物与PCR技术，检测不同地区正常人、高污染区肝细胞癌患者的GSTM1缺失的基因型频率。结果：黄曲霉毒素高危区肝细胞癌患者与正常人的GSTM1基因缺失频率分别为59％和52％，无显著性差异；但与肝细胞癌低发地区正常人相比，其基因缺失频率较高，差异具有显著性意义(P<0.01)。结论：在黄曲霉毒素污染严重地区，单一解毒酶GSTM1的作用难以对抗过量黄典霉毒素B1(AFB1)的致癌活性，其基因缺失不能作为预报肝细胞癌易罹性的独立指标，在筛选易罹者时，必须综合考虑环境致癌物污染浓度、HBV感染及肝细胞损伤程度和多种遗传因素的共同作用。
Objective: To study the relationship between GSTM1 gene polymorphism and high-risk area of aflatoxin in hepatocellular carcinoma, and to verify the reliability of the index as a predictor of hepatocellular carcinoma. METHODS: Genotype frequencies of GSTM1 deletion in normal and highly contaminated hepatocellular carcinoma patients from different regions were detected using specific primers and PCR. RESULTS: The frequency of GSTM1 gene deletion in patients with high-risk aflatoxin and normal controls was 59% and 52%, respectively, with no significant difference. However, the frequency of gene deletion was lower in patients with hepatocellular carcinoma and normal controls than in normal controls with low incidence of hepatocellular carcinoma. High, the difference has significant significance (P <0.01). Conclusion: In the severely contaminated areas of aflatoxin, the effect of the single detoxification enzyme GSTM1 is difficult to counteract the oncogenic activity of excess Xymorphin B1 (AFB1), and its gene deletion cannot be used as an independent indicator to predict the susceptibility of hepatocellular carcinoma. In the case of a person, the combined effects of environmental carcinogen concentration, HBV infection, hepatocyte injury, and multiple genetic factors must be considered.