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从中国林蛙 (Ranachensinensis)、中华大蟾蜍 (Bufogargarizans)以及鳕鱼 (Gadusmacrocephalus)等组织材料中提取DNA ,扩增出约 340bp的 12SrRNA基因片段 ,测定其序列 ,并参考有关序列 ,设计一对哈蟆油的鉴别引物HsmL1、HsmH1,用该鉴别引物扩增蛙类原动物的模板DNA时 ,只有中国林蛙和黑龙江林蛙的模板能得到阳性扩增 ,其余样品为阴性 .因此该对引物能用于哈蟆油药材来源的鉴别 .对购自 5个不同药材商店的哈蟆油PCR鉴定结果表明 ,现市售商品哈蟆油有不同的材料来源 .图 3表 1参 9
DNA was extracted from Ranachensinensis, Bufogargarizans and Gadus macrocephalus. Approximately 340 bp of 12S rRNA gene fragment was amplified, sequence was determined, and a pair of primers was designed with reference to the sequence. The identification primers HsmL1 and HsmH1 of the oil were used to amplify the template DNA of the frog progenitors. Only the templates of Chinese Rana sylvatica and R. henryi were positively amplified, and the remaining samples were negative. Therefore, the paired primers can be used. The identification of the sources of halva oil medicinal materials. The results of PCR identification of hagoil oils purchased from 5 different medicinal herbs shops show that the commercially available hazel oil has different material sources. Figure 3 Table 1 Reference 9